In Depth Characterization with Mass Spectrometry and Orthogonal Methods of Conformers in a Trispecific HIV Antibody
18th November 2020 | 10:00 am CEST | Bruno Genet, Lab head Structural Analysis MS at Sanofi |WATCH FOR FREE
The initial characterization of a trispecific anti-HIV antibody leads to an aberrant two-peaks size exclusion chromatography profile. In such a case, a column interaction is suspected, but the possibility of actually having conformers was taken into consideration. We have used both analytical characterization, structural analysis, and molecular dynamics to cross all the information. The mass spectrometry toolbox has been largely used with native and denaturing MS, ion mobility, and direct infusion to carefully study both the antibody and the antigens interactions.
Presented by Bruno Genet, Lab head Structural Analysis MS at Sanofi
Bruno Genet is currently lab head of the Mass Spectrometry lab in the BioDevelopment department at Sanofi, based in the Paris area. He has broad experience in the Biopharmaceutical Industry, including prior experience at Roussel Uclaf and Aventis. The activities cover the characterization by Mass Spectrometry from the early to the late phase of biotherapeutic projects, up to industrial affairs. Before joining the development of Biologics, he spent several years in a proteomics team to support innovative projects.