DSF and FTIR as complementary methods for the identification and characterization of vaccine products


18th September 2018 | 10 AM UTC | Marina Kirkitadze, Deputy Director, Head of Biophysics & Conformation Unit, Analytical R&D Biochemistry, Sanofi Pasteur and Rina Dukor, President, BioTools |WATCH FOR FREE

The focus of this presentation is the application of Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Fluorimetry (DSF) methods to characterize vaccine components and their stability. Additionally, FTIR can be applied for the identification of final vaccine products, and DSF can be used to distinguish different formulations of vaccine candidates. These methods, when used in conjuction, provide valuable information regarding characterization and stability in the final stages of vaccine manufacturing.

Presented by Marina Kirkitadze, Deputy Director, Head of Biophysics & Conformation Unit, Analytical R&D Biochemistry, Sanofi Pasteur

Marina Kirkitadze is Head of Biophysics and Conformation Unit at Biochemistry Platform, Analytical R&D North America, Sanofi Pasteur Ltd., Toronto, Canada. She has 15 years’ experience in the vaccine industry. Marina received her PhD in Biological Sciences at the Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia, and her MBA from the University of Phoenix, AZ, USA. Marina’s main focus is characterization of vaccine components, specifically, protein conformation and stability in solution and in adjuvanted form.

 

 

 

Sponsored by BioTools

BioTools is known worldwide as the innovator of award-winning disruptive technologies for structural characterization of chiral molecules and biologics.  It was first to commercialize the techniques of VCD (Vibrational Circular Dichroism) and ROA (Raman Optical Activity) and to bring to bio-pharma targeted solutions such as its dedicated protein analyzer PROTA based on FT-IR spectroscopy.  Our unsurpassed knowledge of spectroscopy led to the expansion of our Raman line to include portable instruments for applications such as counterfeit biologics, quality control and formulation.

BioTools’ award-winning ChiralRAMAN ROA spectrometer has recently been shown by Pharma as the most sensitive technique for HOS comparability (innovator / biosimilar or internal such as different lots) and predictor of instability. And since the late 1990’s our PROTA line of FT-IR solutions has been used for structure characterization, formulation and quality control for the vast majority of biologics on the market today.

Our customers include many of the leaders and innovators in the protein therapeutic field.  We pride ourselves on understanding customer goals and problems, training, customer support and products that help solve some of the most challenging industry issues.

Presented by Rina Dukor, President, BioTools

Rina Dukor is the President of BioTools, a company she co-founded with Professor Laurence Nafie in 2000.

Rina received a Ph.D. in physical chemistry from the University of Illinois, Chicago in 1991. Her thesis explored Vibrational Circular Dichroism (VCD) of biological molecules. Upon graduation, Rina joined Amoco’s diagnostic division Vysis (now a part of Abbott) where she established a spectroscopy laboratory focused on proteins and nucleic acids. While in industry, she pioneered the introduction of aqueous infrared spectroscopy to the biopharmaceutical industry through the development of instrumentation, sampling techniques and software for protein secondary structure determination. Her methodology, commercialized as PROTA, is now used by close to a 100 of the leading biopharmaceutical companies. She has pioneered the development of reflection infrared micro- spectroscopy for cancer diagnostics. And by bringing VCD to the market, Rina helped cement the use of VCD by major pharmaceutical companies for the determination of absolute configuration of chiral pharmaceuticals which has truly become one of the most-talked about techniques for chiral analysis.

Rina has co-authored over 60 peer-reviewed papers; several review chapters and is a holder of four patents. Dr. Dukor is a recipient of several awards including Lifetime Achievement Award from the University of Illinois and the William-Wright Award from the Pittsburgh Conference. Rina serves on several Boards including the Board of Visitors for LAS College at UIC, IBC of Scripps Florida and Advisory Boards of companies and scientific organizations.

 


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