Raman spectroscopy measurements during Bioprocessing

11th July 2018 | 10AM EST | Nobel Vale, Research Scientist II, Bristol-Myers Squibb |ATTEND FREE WEBINAR

Raman spectroscopy has been seen as a spectroscopic tool used in bioprocessing to monitor and control cell metabolism to optimize quality and yield of titer. This is due to Raman’s insensitivity to water and ability to analyze samples without manipulation. However there are certain challenges for Raman when analyzing a complex and dynamic sample such as a cell culture. This article will describe the different challenges in analyzing in-line Raman data and how to mitigate changes in samples that include changes in fluorescence background, presence of cells/debris in samples, and spectral interference.

Presented by Nobel Vale, Research Scientist II, Bristol-Myers Squibb

Nobel Vale is a Research Scientist II from Bristol-Myers Squibb since 2012. He was part of the upstream process development group and responsible for optimizing cell culture bioreactor processes to increase productivity. He transitioned internally to the Department of Drug Product Science and Technology and has still works closely with BMS’s upstream groups to enable the use of continuous process monitoring techniques. Some techniques include Raman spectroscopy, capacitance, and automated sampling. Nobel earned his B.S. in Biomedical Engineering from the New Jersey Institute of Technology and M.S. in Biomedical Engineering from Duke University (2009). Prior to BMS, he worked at a start-up called 3D Biotek, a company that develops 3D cell culture devices for stem cell/tissue engineering and drug discovery applications.


Sponsored by Kaiser Optical Systems, Inc

Kaiser Optical Systems Inc. is a world leader in spectrographic instrumentation and applied holographic technology. Principal products include Raman spectroscopy analyzers and fiber-optic sampling probes for examining composition and material characteristics of solids, liquids and gases. Kaiser Raman equipment is deployed in laboratory, analytical and process environments at leading manufacturers throughout the world. Kaiser also produces specialized optics for large telescopes and holography. Since 2013, Kaiser is a wholly-owned subsidiary of Endress+Hauser, which has enhanced Kaiser’s ability to serve its customers globally.