Raman spectroscopy measurements during Bioprocessing
11th July 2018 | 10AM EST | Nobel Vale, Research Scientist II, Bristol-Myers Squibb |WATCH THIS FREE ON DEMAND WEBINAR
Raman spectroscopy has been seen as a spectroscopic tool used in bioprocessing to monitor and control cell metabolism to optimize quality and yield of titer. This is due to Raman’s insensitivity to water and ability to analyze samples without manipulation. However there are certain challenges for Raman when analyzing a complex and dynamic sample such as a cell culture. This article will describe the different challenges in analyzing in-line Raman data and how to mitigate changes in samples that include changes in fluorescence background, presence of cells/debris in samples, and spectral interference.
Presented by Nobel Vale, Research Scientist II, Bristol-Myers Squibb
Nobel Vale is a Research Scientist II from Bristol-Myers Squibb since 2012. He was part of the upstream process development group and responsible for optimizing cell culture bioreactor processes to increase productivity. He transitioned internally to the Department of Drug Product Science and Technology and has still works closely with BMS’s upstream groups to enable the use of continuous process monitoring techniques. Some techniques include Raman spectroscopy, capacitance, and automated sampling. Nobel earned his B.S. in Biomedical Engineering from the New Jersey Institute of Technology and M.S. in Biomedical Engineering from Duke University (2009). Prior to BMS, he worked at a start-up called 3D Biotek, a company that develops 3D cell culture devices for stem cell/tissue engineering and drug discovery applications.
Sponsored by Kaiser Optical Systems, Inc
Kaiser Raman Bioprocessing Solutions
Kaiser Optical Systems, Inc. has been at the leading edge of in situ PAT in upstream bioprocessing for over 10 years. Kaiser Raman technology is robust, scaleable, and transferable. During process development, Raman enables adoption of QbD principles to define manufacturing design spaces, optimize process conditions, and demonstrate process robustness and quality. In production, improvements to product quality and yield have been demonstrated by employing Raman-based control strategies. In recent years, Kaiser Raman successes in upstream bioprocessing have expanded into downstream and cell / gene therapy applications. An overview of biopharma PAT milestones and Kaiser Raman technology advances will be presented.
Presented by Justin Moretto, Bioprocessing Consultant, Kaiser Optical Systems, Inc.
Justin studied neuroscience, biotechnology and physical chemistry at East Carolina University, Johns Hopkins and North Carolina State University. He has worked in worked in novel compound discovery, GMP manufacturing, stem cell development, and bioprocess development. He began working with Kaiser while a scientist at Biogen and subsequently spent 6 years growing the “Raman project” from a development curiosity into a global strategy. Justin joined Kaiser in early 2018 to continue this work.