CELL CULTURE MEDIA IDENTIFICATION IN BIOPHARMACEUTICALS WITH HANDHELD RAMAN
Posted on March 20, 2014
• Improved quality control
• Increased production efficiency
CELL CULTURE MEDIA IDENTIFICATION USING RAMAN ANALYSIS
Cell culture media used for the growth of cells plays an important role in the quality and efficiency of biopharmaceutical production. Raman spectroscopy is a non-destructive, reliable, efficient, and cost effective method to analyze complex cell culture media. Now recognized by the USP and EP as a viable technique for compendial identification, Raman analysis provides highly detailed chemical information on a variety of samples and requires little to no sample preparation.
MINIMIZE FLUORESCENCE WHILE MAXIMIZING EFFICIENCY
Fluorescence generated from cell culture media is a common problem associated with handheld Raman analyzers utilizing a lower excitation wavelength 785nm laser source. With a higher excitation wavelength generated by the handheld Rigaku ProgenyTM 1064nm analyzer, signal blocking fluorescence is minimized. To demonstrate the advantages of the use of a 1064nm analyzer, four synthetic cell culture media were analyzed with Rigaku Raman 1064nm and 795nm spectrometers (Figures 1-4). The 785nm spectra produced significant fluorescence, making it difficult to obtain reliable and specific sample data. In contrast, 1064nm spectra produced compound specific “signature” Raman peaks used to reliably distinguish different media. Spectra in Figure 5 resulted from 1064nm analysis of two cell culture media containing small differences in component structure, both of which are versions of a minimum essential medium developed by Harry Eagle. Clear differences are seen between the Raman signatures. Performance of an on-board spectral comparison resulted in accurate identification of the specific medium.
Progeny 1064nm Advanced Analysis Technology
• Expands the list of identifiable materials
• Distinguishes between closely related compounds
• Generates higher quality data
To learn more please visit us at http://www.rigakuprogeny.com
Related Topics and Keywords
Cell culture media, CELL CULTURE MEDIA IDENTIFICATION, compendial identification, counterfeit, EP, handheld Raman, handheld Raman analyzers, manufacturing, ProgenyTM, Raman spectroscopy, regulatory, USP, Vibrational spectroscopy
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