MycoSEQ™ Mycoplasma Detection Assay – Life Technologies

The MycoSEQ^TM Mycoplasma Detection Assay is a fully integrated solution for qPCR-based mycoplasma detection. For sample preparation the assay utilises novel, proprietary magnetic bead-based DNA purification chemistry. This DNA purification was designed to maximise mycoplasma DNA recovery and minimise the presence of qPCR inhibitors in the test sample. There are multiple sample preparation protocols including a rapid, small-scale protocol suitable for in-process testing, a large scale protocol for cell culture harvest of raw material testing, an automated protocol for high-throughput testing, and specialised protocols for cell and tissue therapy products. For detection of mycoplasma DNA, the MycoSEQ^TM Mycoplasma Detection Assay utilizes SYBR^® Green-based Real-Time qPCR chemistry. This detection chemistry provides multiple advantages including: multi-component, objective result interpretation, no post amplification sample handing and quantitative assessment of the presence of any potential PCR inhibitors in the test sample and a discriminatory positive/extraction control. Extensive bioinformatic analysis was utilised to design a multiplexed primer set for this assay. These primers allow for sensitive, comprehensive detection of more than 90 mycoplasma species, including the Acholeplasma and Spiroplasma species. Additionally, the primers were designed to avoid detection of species closely related to mycoplasma at the genetic level. www.lifetechnologies.com/PharmaAnalytics

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