Downstream Chromatography – GE Healthcare Life Sciences

GE Chromo Efficiency in MAb capture and polishing chromatography steps The use of monoclonal antibodies (MAbs) and MAb conjugates as biopharmaceuticals have increased over the last decade. As a result, more cost-effective, efficient, and flexible process purification solutions are of high priority for MAb manufacturers. Increasing product titers upstream can introduce challenges in downstream purification processes. With increased MAb titers, the cell culture supernatant might contain an elevated number of impurities (e.g., aggregates) that need to be separated from the target molecule. GE Healthcare Life Sciences chromatography media for MAb purification are all based on high-flow agarose base matrices. Protein A media such as MabSelect SuRe™ LX enable efficient capture of MAbs to high purity and yield from high-titer harvested cell culture fluids. The alkali-stabilised protein A ligand permits the use of NaOH for rigorous and cost-effective cleaning operations. Multimodal chromatography media such as Capto™ adhere and Capto adhere ImpRes offer the possibility of two-step purification processes. Traditional ion exchangers such as Capto SP ImpRes and Capto Q media can be combined for efficient removal of remaining impurities in tree-step processes. For efficient process development, GE Healthcare’s selection of chromatography media is available in bulk and in a broad range of scalable formats for HTPD applications. Learn more at

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