Coupling single-pass TFF before loading onto a chromatography sorbent

Process and economic benefits could be achieved by coupling single-pass tangential-flow filtration (SPTFF) and chromatography steps at manufacturing scale. This results in significant sorbent/buffer cost savings and/or reduced processing times. Additionally, the reduced volumes will also help to downsize all future unit operations. As a result, smaller equipment, tank sizes, and hold-up volumes will reduce system footprints. Following application note summarizes the advantages of coupling SPTFF with chromatography unit operations.



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